Molecular Characterization of Fusarium proliferatum and F. equiseti of Pisum sativum Seed

Backgraund: The presence or absence of fungi on the seed surface is an important aspect that determines quality seeds. Since data Fusarium spp. and other seed-borne pathogens vegetable pea in Serbia are scarce, aim this study was to isolate identify disease-causing from infected seed. Methods: Colony morphology microscopic characters isolated species were recorded cultures grown PDA CLA, respectively. In order confirm morphological identification at level, a single-spore culture representative isolates subjected genomic DNA extraction gene amplification using translational elongation factor 1α (TEF-1α) region. PCR products sequenced sequences compared against GenBank nucleotide database by BLAST alignment. Result: Based characteristics, as well molecular sequencing TEF gene, equiseti F. proliferatum confirmed Ps18, Ps19 Ps1, Ps36 obtained seeds, According our knowledge research, first report seeds Serbia.